The granulocyte-macrophage colony-stimulating factor receptor: linking its structure to cell signaling and its role in disease.

Expression of a Chimeric Protein Containing the Catalytic Domain of Shiga-Like Toxin and Human Granulocyte Macrophage Colony-Stimulating Factor (hGM-CSF) in Escherichia coli and Its Recognition by Reciprocal Antibodies

Fusion of two genes at DNA level produces a single protein, known as a chimeric protein. Immunotoxins are chimeric proteins composed of specific cell targeting and cell killing moieties. Bacterial or plant toxins are commonly used as the killing moieties of the chimeric immunotoxins. In this investigation, the catalytic domain of Shiga-like toxin (A1) was fused to human granulocyte macrophage ...

Induction of Apoptosis on K562 Cell Line and Double Strand Breaks on Colon Cancer Cell Line Expressing High Affinity Receptor for Granulocyte Macrophage-Colony Stimulating factor (GM-CSF)

Background: Immunotoxins are comprised of both the cell targeting and the cell killing moieties. We previously established a new immunotoxin, i.e. Shiga toxin granulocyte macrophage-colony stimulating factor (StxA1-GM-CSF), comprises of catalytic domain of Stx, as a killing moiety and GM-CSF, as a cell targeting moiety. In this study, the ability of the immunotoxin to induce apoptosis and dou...

Improvement in Development and Quality of 8 Cell Mouse Embryos in Presence of Granulocyte Macrophage-Colony Stimulating Factor

Purpose: Granulose Macrophage-Colony Stimulating Factor (GM-CSF) is a lympho- heamatopoietic actor, secreted in the reproductive system. Murine pre- implantation embryos express GM-CSF receptors. In his study, the capacity of eight cell mouse embryos was studied in the presence and absence of GM-CSF. Materials and Methods: Female NMRI mice were super ovulated using Pregnant Mare Serum gonadotr...

Comparison of T7- and Lac-Based Systems for the Periplasmic Expression of Human Granulocyte Macrophage Colony Stimulating Factor in Escherichia coli

The GM-CSF receptor: linking its structure to cell signaling and its role in disease

1 Division of Human Immunology, Centre for Cancer Biology, Hanson Institute, Frome Road, Adelaide, South Australia. 2 The Murdoch Children’s Research Institute, Royal Children’s Hospital, Flemington Road, Parkville, Victoria, Australia. 3 St Vincent’s Institute for Medical Research, Princes Street, Fitzroy, Victoria, Australia. Blood First Edition Paper, prepublished online May 12, 2009; DOI 10...